ICS 13.020.01 CCS Z01 团体 标准 T/CSES 80—2023 淡水生物 DNA条形码构建技术规程 Technical regulations for construction of DNA barcodes of freshwater organisms 发布稿 2023 - 01 - 04发布 2023 - 01 - 04实施 中国环境科学学会 发布 全国团体标准信息平台 T/CSES 80 —2023 I 目次 前言 ................................ ................................ ................................ ................................ ................................ ...... II 引言 ................................ ................................ ................................ ................................ ................................ .... III 1 范围 ................................ ................................ ................................ ................................ ................................ 1 2 规范性引用文件 ................................ ................................ ................................ ................................ ............ 1 3 术语和定义 ................................ ................................ ................................ ................................ .................... 1 4 试剂 ................................ ................................ ................................ ................................ ................................ 3 5 设备和材料 ................................ ................................ ................................ ................................ .................... 3 样品采集及前处理设备 ................................ ................................ ................................ ......................... 3 实验室分析设备 ................................ ................................ ................................ ................................ ..... 3 分析软件 ................................ ................................ ................................ ................................ ................. 3 其他辅助设备和材料 ................................ ................................ ................................ ............................. 3 6 DNA 条形码构建方法 ................................ ................................ ................................ ................................ .. 3 DNA条形码构建流程 ................................ ................................ ................................ ........................... 4 物种采集与鉴定 ................................ ................................ ................................ ................................ ..... 4 条形码扩增与测序 ................................ ................................ ................................ ................................ . 4 条形码评估 ................................ ................................ ................................ ................................ ............. 5 物种条形码 ................................ ................................ ................................ ................................ ............. 5 7 质量控制与质量保证 ................................ ................................ ................................ ................................ .... 5 质量控制 ................................ ................................ ................................ ................................ ................. 5 质量保证 ................................ ................................ ................................ ................................ ................. 5 8 废弃物处理 ................................ ................................ ................................ ................................ .................... 6 附录 A（资料性）常用 DNA条形码引物及 PCR扩增条件 ................................ ................................ .......... 7 附录 B（规范性）遗传距离分析原理和方法 ................................ ................................ ................................ .. 9 附录 C（资料性） DNA条形码数据表格 ................................ ................................ ................................ ....... 12 全国团