ISO/TS TECHNICAL SPECIFICATION 23893-2 First edition 2007-11-15 Water quality Biochemical and physiological measurements on fish - Part 2: Determination of ethoxyresorufin-O-deethylase (EROD) Qualite de I'eau Mesurages biochimiques et physiologiques sur poisson- Partie 2: Dosage de I'éthoxyrésorufine-O-dééthylase (EROD) Reference number ISO/TS 23893-2:2007(E) @ ISO 2007 Not for Resale ISO/TS 23893-2:2007(E) PDF disclaimer This PDF file may contain embedded typefaces. In accordance with Adobe's licensing policy, this file may be printed or viewed but shall not be edited unless the typefaces which are embedded are licensed to and installed on the computer performing the editing. In accepts no liability in this area. Adobe is a trademark of Adobe Systems Incorporated. Details of the software products used to create this PDF file can be found in the General Info relative to the file; the PDF-creation parameters were optimized for printing. Every care has been taken to ensure that the file is suitable for use by IsO member bodies. In the unlikely event that a problem relating to it is found, please inform the Central Secretariat at the address given below. COPYRIGHTPROTECTED DOCUMENT @ISO2007 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means electronic or mechanical, including photocopying and microfilm, without permission in writing from either isO at the address below or IsO's memberbody in the country of the requester. ISO copyright office Case postale 56. CH-1211Geneva 20 Tel. + 4122 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland @ ISO 2007 - All rights reserved I without license from IHS Not for Resale ISO/TS23893-2:2007(E) Contents Page Foreword. Introduction 1 Scope 2 Normative references 3 Principle. 4 Test environment. 5 Reagents 6 Apparatus.. 7 Sampling and preparation of samples.... 7.1 Sampling of fish .... 7.2 Killing of fish and dissection. 7.3 Storage. 8 Procedure 8.1 Preparation of the fractions. 8.2 Determination of protein .... 8.3 Determination of the EROD activity.... 8.4 Checking the sensitivity of the biological reagent and compliance with the operating method.. 6 9 Expression of results 9.1 Calculation of the EROD activity.... 9.2 Statistical processing of the data 10 Test report .8 Annex A (informative) Examples of results, 9 Bibliography 14 Copyright International Oorganization fr Standardizalon'ghts reserved ii from IHS Not for Resale ISO/TS23893-2:2007(E) Foreword Iso (the International Organization for Standardization) is a worldwide federation of national standards bodies technicalcommittees.Eachmemberbodyinterestedinasubjectforwhichatechnicalcommitteehasbeen established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with isO, also take part in the work. iso collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/lEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an technical committee may decide to publish other types of normative document: an IsO working group and is accepted for publication if it is approved by more than 50 % of the members of the parent committee casting a vote; anIsOTechnicalSpecification(isO/TS)representsanagreementbetweenthemembersofatechnica committee and is accepted for publication if it is approved by 2/3 of the members of the committee casting a vote. An ISO/PAS or ISO/TS is reviewed after three years in order to decide whether it will be confirmed for a further three years, revised to become an International Standard, or withdrawn.